Attachment of Borrelia burgdorferi within Ixodes scapularis mediated by outer surface protein A
J. Clin. Invest. Utpal Pal, et al. 106:561 doi:10.1172/JCI9427 [Go to this article.]

Figure 2
OspA directly binds to the I. scapularis gut. Direct binding of FITC-labeled OspA (a) to the intact unfixed tick gut was detected using confocal microscopy. FITC-labeled OspC (b), FITC-labeled BSA (c), and FITC (d) were used as controls. After probing the tick gut with FITC-labeled protein (shown in green), the tissues were stained with propidium iodide to localize the nuclei of the gut cells (shown in red). The FITC and propidium iodide images were examined at ×630 and presented as a single image for clarity.