Attachment of Borrelia burgdorferi within Ixodes scapularis mediated by outer surface protein A
J. Clin. Invest. Utpal Pal, et al. 106:561 doi:10.1172/JCI9427 [Go to this article.]

Figure 1
OspA binds to an I. scapularis TGE. (a) FITC-labeled (-F) OspA from B. burgdorferi 25015 or N40, OspC from B. burgdorferi N40, and BSA were used to probe TGE-coated wells. Bars represent the OD₄₅₀ at 15 minutes (mean ± SD) from three experiments. (b) Dose-response curves are presented for FITC-labeled OspA-N40, OspC, and BSA binding to TGE. The OD₄₅₀ was measured at 5 minutes (mean ± SD) from three experiments. (c) FITC-labeled OspA from B. burgdorferi N40 was used to probe TGE-coated, tick salivary gland extract–coated (SG), BSA-coated, gelatin-coated (G), and FCS-coated (FCS) wells. In some studies, TGE was first treated with a protease inhibitor cocktail (TGE-PI) before incubation with OspA. Bars represent the OD₄₅₀ at 15 minutes (mean ± SD) from three experiments.