Sex-related survival differences in murine cardiomyopathy are associated with differences in TNF-receptor expression
J. Clin. Invest. Toshiaki Kadokami, et al. 106:589 doi:10.1172/JCI9307 [Go to this article.]

Figure 5
Cytokine-receptor expression in peripheral white blood cells and liver tissues. Representative images and quantitative results of RNase protection assays for TNFR1 and TNFR2 in peripheral white blood cells (a–c) and liver tissues (d–f). Quantitative results are reported as the ratio of the pixel intensity of each protected probe normalized to that of the GAPDH probe included in each template set as an internal control, as described in Methods. Although TNF1.6 mice expressed significantly more TNFR1 and TNFR2 RNA in peripheral white blood cells, there was no significant difference between sexes in cytokine-receptor gene expression in TNF1.6 and wild-type mice (a–c). Transcript level of cytokine receptors in the liver was comparable in males and females in both TNF1.6 and wild-type mice (d–f). Values are mean ± SD. Y-axis values correspond to the signal intensity of the hybridized probe in pixels normalized to that of GAPDH. TG, TNF1.6 mice; WT, wild-type mice; M, male; F, female. ^AP < 0.001 vs. WT of the same sex.