Thrombin activates a Y box–binding protein (DNA-binding protein B) in endothelial cells
J. Clin. Invest. Olga I. Stenina, et al. 106:579 doi:10.1172/JCI9075 [Go to this article.]

Figure 3
Comparison of truncated dbpB to TINF. A truncated dbpB cDNA was prepared by introducing a stop codon following position 621 and subcloning into the pcDNA3 vector. The truncated dbpB cDNA was transcribed and translated in vitro and subjected to SDS-PAGE (8% gel) following cross-linking to the DNA probe (a) and EMSA (b). (The ThRE oligonucleotide was used as a probe in both assays). pcDNA3, empty pcDNA3 vector used as a control for the in vitro transcription and translation reaction; truncated dbpB, truncated dbpB cDNA in pcDNA3 vector used for an in vitro translation reaction; contro, extract from nonstimulated ECs; thrombin, extract from thrombin-stimulated ECs (10 U/mL for 2 hours).