Thrombin activates a Y box–binding protein (DNA-binding protein B) in endothelial cells
J. Clin. Invest. Olga I. Stenina, et al. 106:579 doi:10.1172/JCI9075 [Go to this article.]

Figure 1
Localization of full-length dbpB in endothelial cells with and without thrombin treatment. Bovine ECs were transiently transfected with a cDNA encoding a fusion protein of full-length dbpB and green fluorescent protein, as described in Methods. (a) Without thrombin stimulation. (b) After 2-hour stimulation with thrombin (10 U/mL). Nuclei were stained with propidium iodide. (c) FACS quantitation of this experiment. Nuclei were isolated from nonstimulated and thrombin-stimulated ECs (10 U/mL for 2 hours) and analyzed by FACS. Arbitrary units are used for both the number of GFP-containing nuclei and mean intensity of fluorescence of the nuclei (n = 10; P < 0.01).