Inhibition of T lymphocyte activation in mice heterozygous for loss of the IMPDH II gene
J. Clin. Invest. Jing Jin Gu, et al. 106:599 doi:10.1172/JCI8669 [
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Figure 3PCR genotyping of mouse blastocysts. Blastocysts were isolated from
IMPDH II+/– breeding females at 3.5 dpc. PCR was performed using two sets of oligonucleotide primers. Lane 1 contains molecular weight markers of lambda HindIII plus PhiX174 Hae III. Lanes 2–4 are examples of heterozygotes, since they have both wild-type (WT) and knock-out (KO) bands; lanes 5 and 6 are examples of wild-type; and lanes 7 and 8 are examples of homozygotes. Lane 9 contains tail DNA from an IMPDH II heterozygous animal, and lane 10 represents a PCR reaction in the absence of DNA.
