Retinal expression, regulation, and functional bioactivity of prostacyclin-stimulating factor
J. Clin. Invest. Yasuaki Hata, et al. 106:541 doi:10.1172/JCI8338 [
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Figure 4PSF mRNA expression is increased by cell confluency. (
a) REC RNA was isolated at 80% cell confluency (sub) and at 0, 1, 3, and 5 days after confluency (Cd) was achieved.
AP < 0.012. (
b) REC RNA was isolated after 24 hours’ exposure to media containing 0.1% BSA or calf serum (CS) at the percentages indicated in the figure.
AP < 0.001. Representative Northern blot analysis for PSF (top), quantitation from multiple experiments after normalization to 36B4 control signal (middle), and representative Northern blot analysis of TGF-β RNA expression in the same cells (bottom) are presented.
