Retinal expression, regulation, and functional bioactivity of prostacyclin-stimulating factor
J. Clin. Invest. Yasuaki Hata, et al. 106:541 doi:10.1172/JCI8338 [Go to this article.]

Figure 3
Cytokines and hypoxia associated with worsening of diabetic retinopathy increase PSF expression. (a) Confluent cultures of RECs were exposed to TGF-β (1 ng/mL), VEGF (25 ng/mL), bFGF (10 ng/mL), or hypoxia (0.5% O₂, 5% CO₂, 94.5% N₂) for 24 hours before evaluation of the media for PSF protein by Western blot analysis. T, TGF-β; V, VEGF; F, bFGF. (b) Nearly confluent cultures of RECs were exposed to hypoxic conditions (0.5% O₂, 5% CO₂, 94.5% N₂) for the period of time indicated in the figure before isolation of RNA and Northern blot analysis. Cells were either isolated at the same time after initial plating (open bars) or sequentially after plating (filled bars). Representative Northern blot analysis (top) and quantitation (bottom) are presented.