Satellite cells are a stem cell population within adult muscle and are responsible for myofiber regeneration upon injury. Satellite cell dysfunction has been shown to underlie the loss of skeletal muscle mass in many acquired and genetic muscle disorders. The transcription factor paired box-protein-7 (PAX7) is indispensable for supplementing the reservoir of satellite cells and driving regeneration in normal and diseased muscle. TNF receptor–associated factor 6 (TRAF6) is an adaptor protein and an E3 ubiquitin ligase that mediates the activation of multiple cell signaling pathways in a context-dependent manner. Here, we demonstrated that TRAF6-mediated signaling is critical for homeostasis of satellite cells and their function during regenerative myogenesis. Selective deletion of
Sajedah M. Hindi, Ashok Kumar
Expression of TRAF6 in satellite cells.
Primary mononucleated cells were isolated from hind limb muscle of WT mice and subjected for FACS analysis for the expression of α7-integrin and TRAF6, either immediately after isolation (quiescent) or after purification and culturing (activated). (