Myeloid dendritic cells induce Th2 responses to inhaled antigen, leading to eosinophilic airway inflammation
J. Clin. Invest. Bart N. Lambrecht, et al. 106:551 doi:10.1172/JCI8107 [Go to this article.]

Figure 4
Measurement of cytokine levels in unconcentrated BALF and after in vitro restimulation of LN cells with specific antigen. Mice were treated as in Figure 2. Groups are coded as immunization/exposure. (a) BALF was taken 24 hours after the last aerosol exposure and assayed for the presence of IL-4 and IFN-γ by ELISA. IL-5 could not be detected in BALF. (b and c) Mediastinal and inguinal LNs were resected, and single-cell suspensions were cultured in vitro for 96 hours in the presence of OVA. Results represent means ± SEM from five to eight animals per group.