Lnk controls mouse hematopoietic stem cell self-renewal and quiescence through direct interactions with JAK2
J. Clin. Invest. Alexey Bersenev, et al. 118:2832 doi:10.1172/JCI35808 [Go to this article.]

Figure 3
Lnk^–/– HSCs have an increased quiescent HSC population compared with WT HSCs. (A) WT, Mpl^–/–, Lnk^–/–, and Mpl^–/–Lnk^–/– mice were fed with water containing BrdU for 12 days. Left panels show representative FACS plots of LSK cells gated for Flk2 surface expression. The solid line indicates the reduction staining control, which contains all fluorochromes except Flk2-PE. Shaded areas indicate Flk2 antibody staining. Numbers indicate the percentage of cells represented by the solid line and shaded area. Bold numbers indicate that WT and Mpl^–/– mice were significantly different from Lnk^–/– mice. BrdU incorporation analysis in purified Flk2^–LSK (middle panels) and Flk2⁺LSK (right panels) HSCs were determined by FACS. Representative pseudocolor plots are shown (mean ± SD). n = 4. For middle and right columns, percentages indicate the percentage of cells gated in the respective quadrants. Bold numbers indicate that Lnk^–/– and Mpl^–/– mice were significantly different from WT mice. (B) Sorted Flk2^–LSK HSCs were stained with Py and Ho, and representative contour plots are shown. The quiescent G₀ populations defined as Py^–Ho^– are indicated (mean ± SEM). n = 4.