Lnk controls mouse hematopoietic stem cell self-renewal and quiescence through direct interactions with JAK2
J. Clin. Invest. Alexey Bersenev, et al. 118:2832 doi:10.1172/JCI35808 [Go to this article.]

Figure 2
Lnk^–/– LSK HSCs show decelerated cell cycle kinetics. WT, Mpl^–/–, Lnk^–/–, and Mpl^–/–Lnk^–/– mice were fed with water containing BrdU for 7 days. Left panels show representative FACS plots of Lin^–Kit^hi BM cells gated for LSK and LK HSC progenitors. Numbers indicate percentage of cells within each boxed area. Bold numbers indicate that Lnk^–/– and Mpl^–/– mice were significantly different from WT mice. BrdU incorporation analysis in purified LSK (middle panels) and LK (right panels) cells were determined by FACS. Representative pseudocolor plots are shown. BrdU^– 2N, BrdU⁺ 2N, and BrdU⁺ populations with over 2N DNA content were quantified (mean ± SD). n = 4.