Multidrug resistance-associated protein 4 regulates cAMP-dependent signaling pathways and controls human and rat SMC proliferation
J. Clin. Invest. Yassine Sassi, et al. 118:2747 doi:10.1172/JCI35067 [
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Figure 4Use of Ad-shMRP4 in vivo. (
A) Map of the Ad-shMRP4 vector and the MRP4 shRNA sequence. (
B) Quantitative real-time PCR and (
C) western blot of rat SMCs transfected for 72 h with Ad-shMRP4 or Ad-shLuc (
n = 3, ***
P < 0.001). Viruses were used at an MOI of 30. (
D) Representative hematoxylin and eosin–stained sections of non-injured and injured carotid arteries infected with Ad-shLuc or Ad-shMRP4 14 days after surgery. Scale bar: 50 μm. (
E) Average intima/media thickness ratios for the above 3 groups (*
P < 0.05, ***
P < 0.001 compared with Ad-shLuc). ad, adventitia (
n = 5 for non-injured carotid,
n = 4 for Ad-shLuc, and
n = 6 for Ad-shMRP4). (
F) PCR on carotid arteries. DNA from rat carotids was extracted and adenovirus DNA was detected by PCR.
