Multidrug resistance-associated protein 4 regulates cAMP-dependent signaling pathways and controls human and rat SMC proliferation
J. Clin. Invest. Yassine Sassi, et al. 118:2747 doi:10.1172/JCI35067 [Go to this article.]

Figure 3
Inhibition of hCASMC proliferation with MRP4 siRNA. Quantitative real-time PCR with gene-specific primers of (A) MRP4 and (B) MRP5 in hCASMCs transfected for 72 h with MRP4, MRP5, or scrambled siRNA (n = 3, ***P < 0.001). (C) Western blot analysis of total lysates of hCASMCs transfected for 72 h with MRP4 siRNA or scrambled siRNA, showing efficient silencing of MRP4. Proteins were incubated with anti-MRP4 or anti-PP2B antibodies. PP2B was used as a loading control. n = 3; P ≤ 0.001. (D) Effect of MRP4 and MRP5 siRNAs on hCASMC proliferation (assessed by BrdU incorporation) compared with scrambled siRNA. Proliferation was normalized to the value obtained with 0.1% S. Five experiments were performed in triplicate. ***P ≤ 0.001 for MRP4 compared with scrambled or MRP5 siRNA.