Sex differences in thrombosis in mice are mediated by sex-specific growth hormone secretion patterns
J. Clin. Invest. Joshua H. Wong, et al. 118:2969 doi:10.1172/JCI34957 [Go to this article.]

Figure 5
Expression of coagulation factor and inhibitor genes. Total RNA was prepared from the livers of 4 animals from each group. cDNA was reverse transcribed and subjected to quantitative real-time PCR. Abundance of each mRNA relative to internal controls was calculated to generate gene copy number (GCN) (59). GCN was normalized to the relevant male control (black) and expressed as mean (±SEM) percentage normalized expression. (A) Procoagulant gene expression was not different in female versus male liver. (B) Expression of the inhibitors Proc, Serpind1, and Serpinc1 was increased, while expression of Serpina5 was decreased, in female versus male liver. (C) Expression of Proc, Serpind1, and Serpinc1 was increased and expression of Serpina5 was decreased in female lit^m/+ –pGH and female and male lit^m/m –pGH mice relative to lit^m/+ –pGH. Expression was restored to baseline in female and male lit^m/m +pGH animals. (D) Animals were given cGH as in Figure 3B. Expression of Proc, Serpind1, and Serpinc1 was increased and expression of Serpina5 was decreased in female control mice (WT–cGH) and female and male mice receiving cGH (WT +cGH) relative to male mice –cGH. (E) Animals were given pGH as in Figure 3C. Expression of Proc, Serpind1, and Serpinc1 was decreased and expression of Serpina5 was increased in female control mice (WT –pGH) and female and male mice receiving pGH (WT +pGH) relative to male control WT –pGH mice. *P < 0.01, **P < 0.001, ***P < 0.0001; ANOVA with Bonferroni’s post-hoc test.