Sex differences in thrombosis in mice are mediated by sex-specific growth hormone secretion patterns
J. Clin. Invest. Joshua H. Wong, et al. 118:2969 doi:10.1172/JCI34957 [Go to this article.]

Figure 4
Procoagulant factor activity levels are higher in male than female mice and are GH dependent. Blood was collected as in Figure 1. Coagulation factor activity assays were adapted for use in mice. (A) Plasma from 4 individual male and female mice was mixed with the indicated human factor–deficient plasma, and factor activity was normalized to that in WT B6 males and expressed as the mean (±SEM) percentage of male activity. Factor activity levels are greater in male versus female plasma for all assays (*P < 0.05, **P < 0.001; ANOVA with Bonferroni’s post-hoc test). (B) Plasma from 4 animals of each indicated group was collected and prepared and mean (±SEM) activity levels expressed as a percentage of male lit^m/+ –pGH activity. Factor activity levels were generally lower in female lit^m/+ –pGH and male and female lit^m/m –pGH mice versus male lit^m/+ –pGH and male and female lit^m/m +pGH for all assays. (C) The average of all procoagulant factor activity levels for each group. Factor activity levels were significantly lower in female lit^m/+ –pGH and male and female lit^m/m –pGH versus male lit^m/+ –pGH and male and female lit^m/m +pGH. **P < 0.01, ***P < 0.0001; ANOVA with Bonferroni’s post-hoc test.