Sex differences in thrombosis in mice are mediated by sex-specific growth hormone secretion patterns
J. Clin. Invest. Joshua H. Wong, et al. 118:2969 doi:10.1172/JCI34957 [Go to this article.]

Figure 1
Sex differences in thrombosis in WT mice. Blood was drawn from 15 male and female WT B6 mice. (A) Mean whole-blood clotting times were significantly shorter in male versus female mice. P < 0.0001, Student’s t test. (B) Whole-blood clotting was triggered with 2 dilutions (indicated) of thromboplastin. The reaction was quenched at 5-minute intervals, and thrombin-antithrombin (TAT) levels were measured. For the 1:1,000 dilution, TAT values (±SEM) were higher in male versus female mice at all time points. For the 1:100,000 dilution, TAT was higher in males at the 15- and 20-minute time points (**P < 0.01, ***P < 0.001; ANOVA with Bonferroni’s post-hoc test). Data represent pooled blood from 5 mice of each sex each run in duplicate and measured in duplicate. (C) The indicated numbers of male and female WT mice were injected with 2 μl/g of a 1:160 dilution of thromboplastin in the in vivo model of PE. Data are presented as percent survival, which was greater in female versus male mice as compared by the log-rank test (P = 0.01).