KLF6-SV1 overexpression accelerates human and mouse prostate cancer progression and metastasis
J. Clin. Invest. Goutham Narla, et al. 118:2711 doi:10.1172/JCI34780 [Go to this article.]

Figure 4
Intracardiac model of PCa dissemination and metastasis. (A) Female nu/nu mice were injected with 1 × 10⁶ PC3 cells in 25 μl PBS. In vivo BLI was performed weekly 10–15 minutes after animals received the substrate d-luciferin at 150 mg/kg in PBS by i.p. injection. The bioluminescent signals from metastatic lesions in the KLF6-SV1–expressing PC3 cell group was 3 orders of magnitude higher than the control pBABE vector–expressing PC3 cell–injected mice, demonstrating that the former developed larger metastatic tumors. (B) pSV1 vector–expressing cells demonstrated detectable metastasis significantly earlier than did control pBABE vector–expressing cells lines (P < 0.01). The median time to metastatic development was 3.5 times more rapid in the mice with KLF6-SV1–expressing PC3 cells (7 d) compared with controls (24 d; P < 0.009). (C) Ex vivo imaging and histology was performed on 20 different tissues excised from a subset of mice after final in vivo imaging. Representative images are shown.