KLF6-SV1 overexpression accelerates human and mouse prostate cancer progression and metastasis
J. Clin. Invest. Goutham Narla, et al. 118:2711 doi:10.1172/JCI34780 [
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Figure 3Overexpression of KLF6-SV1 in an orthotopic mouse model of PCa progression results in increased metastasis. (
A) Male SCID beige mice were anesthetized, and the dorsolateral aspect of the prostate was injected with 1 × 10
6 PC3 cells in 25 μl PBS. Tumors were imaged every week to determine local tumor growth and evidence of tumor cell dissemination. A representative image of 2 mice is illustrated. Local tumor growth, as determined by fluorescent intensity, was equal between control and KLF6-SV1 mice. (
B and
C) KLF6-SV1–overexpressing cells metastasized more frequently than did control cells. The number of metastatic lesions was determined using a combination of whole-body imaging and ex vivo histological analysis of all mice upon sacrifice (
n = 8 [pBABE]; 9 [pSV1]); representative images of ex vivo tissue analysis are shown. (
D) pSV1 vector–derived tumors expressed significantly less NOXA and p21 than did control tumors. qRT-PCR of pSV1 vector–derived tumors using real-time primers specific to NOXA, KLF6-SV1, and p21 demonstrated marked overexpression of KLF6-SV1 in pSV1 vector–derived tumors with concomitant reduction in p21 and NOXA expression. **
P < 0.01, ***
P < 0.001 versus control.
