Coxsackievirus and adenovirus receptor (CAR) mediates atrioventricular-node function and connexin 45 localization in the murine heart
J. Clin. Invest. Byung-Kwan Lim, et al. 118:2758 doi:10.1172/JCI34777 [
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Figure 6Abnormal intercalated disc proteins and cardiomyopathy in CAR-cKO mice. (
A) Intercalated disc protein levels in 8-week-old WT and CAR-cKO mouse ventricles by immunoblot. β-catenin and ZO-1 were significantly reduced in mutants (
n = 3 for each group, *
P < 0.01), as quantified by densitometry. (
B) Immunofluorescent stains illustrating the localization pattern of intercalated disc proteins. Scale bar: 40 μm. (
C) 8-week-old mouse hearts were costained for β-catenin (green) and CAR (red) (upper panels), or vinculin (green) and ZO-1 (red) (lower panels). Localization of β-catenin and ZO-1 at the intercalated disc was abnormal in CAR-cKO mice. Scale bar: 20 μm. (
D) Representative electron micrographs from the ventricular myocardium of WT and CAR-cKO mice at 8 weeks of age. Scale bars: 1.4 μm (upper panels); 0.36 μm (lower panels).
