Coxsackievirus and adenovirus receptor (CAR) mediates atrioventricular-node function and connexin 45 localization in the murine heart
J. Clin. Invest. Byung-Kwan Lim, et al. 118:2758 doi:10.1172/JCI34777 [
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Figure 4CAR expression in AV node of WT mice and intact AV-node structure in WT and CAR-cKO mice. (
A and
B) GFP nuclear staining in HCN4-GFP knockin mouse. (
A) Section through the right atria (RA) and left atria (LA) shows the AV node in
B. (
B) Higher power view of the AV node (AVN). (
C) High-power view from HCN4-GFP knockin mouse (green nuclear stain) at the region of the AV node. Scale bars are measured in micrometers. (
D) Immunofluorescent staining for CAR (red, indicated by arrows). (
E) Merged images of
D and
E demonstrate that CAR is located between GFP-positive AV-nodal cells (arrows). Lower panels in
C,
D, and
E show different areas from the same mouse. (
F) Acetylcholinesterase stain of the WT and CAR-cKO mice demonstrate similar staining patterns in the AV node. Lower panels show enlarged photographs of the insets.
