Coxsackievirus and adenovirus receptor (CAR) mediates atrioventricular-node function and connexin 45 localization in the murine heart
J. Clin. Invest. Byung-Kwan Lim, et al. 118:2758 doi:10.1172/JCI34777 [
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Figure 1Generation of
CAR-cKO mice.
(
A) Targeting strategy for deletion of
CAR. Exon 2 of the murine
CAR gene was flanked by loxP sites, and a neomycin (Neo) resistance cassette was inserted downstream, flanked by FRT sites. Sa, SalI; Nh, NheI; N, NotI. (
B) Genomic Southern blot analysis of mutant floxed allele (M) or WT ES cells using probe shown in panel A, after digestion of genomic DNA with NheI. (
C) PCR of WT, floxed heterozygous (H), or homozygous (M) mutant mice from extracted mouse-tail genomic DNA using primers P1 and P2. (
D) Disruption of CAR expression in WT or CAR-cKO mice as measured by immunofluorescent staining and immunoblotting using an antibody specific for CAR (red) and γ-catenin (green) as marker of the cell-cell junction. The typical intercalated disc staining for CAR is noted in the WT mice (arrows) but absent in the CAR-cKO mice. Scale bar: 40 μm.
