CD133 expression is not restricted to stem cells, and both CD133⁺ and CD133^– metastatic colon cancer cells initiate tumors
J. Clin. Invest. Sergey V. Shmelkov, et al. 118:2111 doi:10.1172/JCI34401 [Go to this article.]

Figure 6
Metastatic colon cancer comprises both EpCAM⁺CD133⁺ and EpCAM⁺CD133^– tumor cells. Costaining of colon cancer metastases to the liver with anti-EpCAM (green) and anti-CD133 (red) antibodies. (A and C) Typical examples of metastatic colon cancer harboring 2 subpopulations of tumor cells, EpCAM⁺CD133⁺ and EpCAM⁺CD133^–. (B and D) Metastatic colon cancer completely lacking expression of CD133. The arrows indicate CD133 expression. Scale bar: 50 μm.