Activation of hypothalamic S6 kinase mediates diet-induced hepatic insulin resistance in rats
J. Clin. Invest. Hiraku Ono, et al. 118:2959 doi:10.1172/JCI34277 [
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Figure 6Hypothalamic overexpression of CA-S6K blunts Akt phosphorylation. After 5 h of fasting, 4 μU insulin was infused into the MBH, and 15 min later, the MBH was harvested and analyzed. MBH lysates were blotted with anti–phospho-Thr308 and anti–phospho-Ser473 Akt antibodies. Results are shown as representative bands with quantitation. **
P < 0.01.
