Activation of hypothalamic S6 kinase mediates diet-induced hepatic insulin resistance in rats
J. Clin. Invest. Hiraku Ono, et al. 118:2959 doi:10.1172/JCI34277 [
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Figure 2HFD feeding for 1 d induces insulin resistance in the brain-liver circuit. Rats were fed NC or HFD (171% of the energy of NC) for 1 d. After 5 h of fasting, vehicle (veh) or 4 μU insulin (ins) was infused into the MBH for the entire 6-h duration of the clamp study. (
A) Clamp protocol. Equilibration period, 0–120 min; basal period, 120–240 min; insulin clamp period, 240–360 min, during which systemic insulin and glucose were continuously infused and the rate of the latter was adjusted to maintain euglycemia. (
B) Glucose infusion rate required to maintain euglycemia during the clamp period. (
C) HGP during the basal and clamp periods. (
D) Clamp/basal HGP suppression ratio. (
E) Peripheral glucose uptake during the clamp period. *
P < 0.05; **
P < 0.01; ***
P < 0.001.
