Host-derived oxidized phospholipids and HDL regulate innate immunity in human leprosy
J. Clin. Invest. Daniel Cruz, et al. 118:2917 doi:10.1172/JCI34189 [Go to this article.]

Figure 5
Oxidized phospholipids alter TLR2/1 activation. (A) Monocytes activated with 10 μg/ml TLR2/1 ligand (TLR2/1L) 19 kDa in the presence of PEIPC produced less IL-12 p40, but more IL-10, than those activated with TLR2/1 ligand alone, as determined by ELISA performed in triplicate wells. Data (mean ± SEM) are representative of 2 experiments. (B) Effect of PEIPC on TLR2/1-mediated induction of CYP27b1 mRNA. Monocytes were activated by TLR2/1 ligand in 5% FCS in the presence or absence of PEIPC for 24 h, and CYP27b1 mRNA levels were determined by quantitative PCR. Data are mean ± SEM of 4 separate experiments. *P < 0.03 versus media. (C) Effect of PEIPC on TLR2/1-mediated induction of cathelicidin mRNA. Monocytes were activated by TLR2/1 ligand in 10% human serum (vitamin D sufficient) in the presence or absence of oxPAPC for 24 h, and cathelicidin mRNA levels were determined by quantitative PCR. Data are mean ± SEM of 3 separate experiments. **P = 0.02 versus media.