Host-derived oxidized phospholipids and HDL regulate innate immunity in human leprosy
J. Clin. Invest. Daniel Cruz, et al. 118:2917 doi:10.1172/JCI34189 [Go to this article.]

Figure 4
Oxidized phospholipids alter DC differentiation and function. (A) oxPAPC was separated by HPLC, and 1-minute fractions were collected and individually tested for their effects on CD1b and MHC class II expression on differentiating DCs. Chromatograms demonstrating which fractions contained PGPC, POVPC, and PEIPC have been superimposed with biological activity. (B) Representative fractions enriched in PEIPC (fraction 23), POVPC (fraction 20), and PGPC (fraction 19) differentially affected CD1b expression on differentiating DCs. GM, GM-CSF; FSC, forward scatter; SSC, side scatter. Numbers within plots denote percent cells within gate or positive for CD1b. (C) PEIPC altered DC function. After 48 h differentiation in the presence or absence of 500 ng/ml PEIPC, cells were washed, irradiated, pulsed with antigen, and cocultured with T cell lines. CD1b- or MHC class II–restricted T cell activation was assessed by IFN-γ production. Data (mean ± SEM of triplicate wells) are representative of 3 experiments for CD1b-restricted T cells, 3 experiments for MHC class II–restricted T cells with GroES protein, and 2 experiments using GroES peptide. *P < 0.03 versus GM alone.