Host-derived oxidized phospholipids and HDL regulate innate immunity in human leprosy
J. Clin. Invest. Daniel Cruz, et al. 118:2917 doi:10.1172/JCI34189 [
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Figure 3Oxidized phospholipids accumulate in L-lep lesions and during mycobacterial infection. (
A) Human leprosy lesions were labeled with the monoclonal antibody EO6. Shown are representative sections from L-lep (
n = 5) and T-lep (
n = 4) lesions. Original magnification, ×40. (
B) TUNEL in leprosy lesions (
n = 4 per group). Original magnification, ×20. (
C) Immunofluorescence 3-color confocal microscopy of L-lep lesions. Red stain, EO6; green stain, CD68; blue stain, DAPI. Original magnification, ×63. (
D) oxLDL, but not
M. leprae sonicate, reacted with EO6, as determined by ELISA. Data (mean ± SEM) are representative of 4 independent experiments. (
E) Primary human macrophages accumulated PEIPC during BCG infection. Phospholipids were analyzed by ESI-MS, and relative abundance was determined by comparison with levels of native PAPC. Data are mean ± SEM of 3 independent experiments. *
P < 0.001 versus media.
