Claudin-16 and claudin-19 interact and form a cation-selective tight junction complex
J. Clin. Invest. Jianghui Hou, et al. 118:619 doi:10.1172/JCI33970 [Go to this article.]

Figure 2
CLDN16 interacts with CLDN19 in yeast. (A) Y2H assays showing interaction of CLDN16-WT with CLDN19-WT and CLDN19-WT with CLDN19-WT, but not of CLDN16-WT with CLDN16-WT. Shown are plates with selective medium lacking leucine and tryptophan (–LW), indicating the transforming of both bait and prey vectors; with SD-LWHA, indicating the expression of reporter genes HIS3 and ADE2; and β-galactosidase assay (A615 values) for quantification of interaction strength. (B) Mutations in CLDN19 affecting its homomeric interaction. (C) Mutations in CLDN19 affecting its heteromeric interaction with CLDN16. (D) Mutations in CLDN16 affecting its heteromeric interaction with CLDN19.