Erythropoietin deficiency decreases vascular stability in mice
J. Clin. Invest. Jing Chen, et al. 118:526 doi:10.1172/JCI33813 [Go to this article.]

Figure 5
Epo-induced increase of EPC and microglia numbers in the retina. (A) Representative images of retinal flat mount from P8 oxygen-treated mice with Epo injection (i.p., 5,000 U/kg, P6 and P7) or PBS control stained with CD34 antibody (green) and lectin GS-IB4 (red), with a portion of the image enlarged (white frames). White arrows indicate CD34⁺ cells. (B) Mean number of EPCs (CD34⁺ cells) per vessel length in Epo treated compared with control retina (n = 6 per group; ***P ≤ 0.001). (C) Representative images of retinal flat mount from P8 oxygen-treated mice with Epo injection (i.p., 5,000 U/kg, P6 and P7) or PBS control stained with Csf-1R antibody (green) and lectin GS-IB4 (red). (D) Mean number of microglia (Csf-1R⁺ cells) in Epo-treated versus control retinas (n = 10 per group; **P ≤ 0.005). Original magnification, ×20.