Erythropoietin deficiency decreases vascular stability in mice
J. Clin. Invest. Jing Chen, et al. 118:526 doi:10.1172/JCI33813 [Go to this article.]

Figure 4
Localization of Epo and Epo receptors and Epo-induced NF-κB activity in the retina. (A) Representative retinal cross-sections from P8 normoxia mouse immunolabeled with Epo antibody (red) and lectin (green). (B) Dehydrated retinal cross-section from P8 normoxia retina stained with lectin (green) and counter-stained with H&E for laser capture microdissection. (C) mRNA expression of Pecam, Epo, Epo receptor (Epo-R), and β-common receptor (βCR) in laser-captured retinal cell layers (n = 6 per group). GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer. (D) Real-time PCR quantification of Vegf and Vegf receptor (Flk-1, Flt-1, Nrp1, Hif1a, and Hif2a) mRNA in retina of mouse littermate with Epo treatment (P6 and P7) or PBS control (n = 6 per group). Copy number of mRNA/10⁶ copies cyclophilin A control mRNA were measured at P8 (n = 8 per group). (E) P8 oxygen-treated retinas from NF-κB–Luc reporter mice with PBS (n = 6) or Epo treatment (i.p., 5,000 U/kg, P6 and P7) (n = 7) showing NF-κB–Luc activity (*P ≤ 0.05). (F) Cross-section of P8 oxygen-treated retinas from NF-κB–Luc reporter mice stained with lectin GS-IB4 (red) and luciferase antibody (green) showing NF-κB localization. Original magnification, ×40.