Erythropoietin deficiency decreases vascular stability in mice
J. Clin. Invest. Jing Chen, et al. 118:526 doi:10.1172/JCI33813 [
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Figure 3Early Epo treatment protects retinal neurons from hypoxia-induced apoptosis. (
A) TUNEL (green) and DAPI (red) staining of retinal cross-sections from P17 normoxia mice and oxygen-treated mice with early Epo injection (i.p., 5,000 U/kg, at P6, P8, P10, and P12) or PBS control. A portion of the image from P17 oxygen-treated PBS control retina was enlarged (white frame) in lower panels. Original magnification, ×40. (
B) Quantification of the fluorescence intensity of TUNEL stain in P17 normoxia mice and P17 hypoxia mice with Epo treatment or PBS control (
n = 3 per group; *
P ≤ 0.05). (
C) Caspase-3/-7 activity in retinal homogenate isolated from P17 normoxia mice and P17 hypoxia mice with Epo treatment or PBS control (
n = 6 per group; ***
P ≤ 0.001).
