Erythropoietin deficiency decreases vascular stability in mice
J. Clin. Invest. Jing Chen, et al. 118:526 doi:10.1172/JCI33813 [
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Figure 1Epo treatment prevents oxygen-induced retinal vessel loss in a dose-dependent manner. (
A) Real-time PCR quantification of
Epo mRNA expression in age-matched mouse retinas under normoxia or hyperoxia treatment; copy number of
Epo mRNA/10
6 copies of cyclophilin A control mRNA at P8, P12, P15, and P17 (
n = 6 per group). (
B) Representative retinal whole-mounts showing area of vaso-obliteration after 18 h of oxygen exposure and i.p. injection (P6 and P7) of Epo (right) or saline control (left). Original magnification, ×5. (
C) Dose response of Epo protection against retinal vaso-obliteration at P8 (with i.p. injections at P6 and P7) (saline,
n = 40; Epo 1,000 U/kg,
n = 12; 2,500 U/kg,
n = 7; 5,000 U/kg,
n = 16) **
P ≤ 0.01; ***
P ≤ 0.001.
