Keratinocyte-specific Smad2 ablation results in increased epithelial-mesenchymal transition during skin cancer formation and progression
J. Clin. Invest. Kristina E. Hoot, et al. 118:2722 doi:10.1172/JCI33713 [
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Figure 5Human skin SCCs with Smad2 loss correlated with E-cadherin loss and nuclear Snail. Skin SCCs were stained for Smad2 IHC (brown, top row), and immunofluorescence staining was performed for E-cadherin (green; middle row) and Snail (green; bottom row). A K14 antibody was used for immunofluorescent counterstain (red). An example of a pair SCCs from serial sections showed that a Smad2-positive SCC retained membrane-associated E-cadherin with a few Snail nuclear staining cells. In contrast, SCC with Smad2 loss lost membrane-associated E-cadherin but uniformly expressed Snail in the nucleus. Scale bar: 100 μm.
