IL-22 is required for Th17 cell–mediated pathology in a mouse model of psoriasis-like skin inflammation
J. Clin. Invest. Hak-Ling Ma, et al. 118:597 doi:10.1172/JCI33263 [Go to this article.]

Figure 6
IL-22 induces antimicrobial peptide and proinflammatory cytokine gene expression and keratinocyte hyperplasia in the skin. Ears of BALB/c mice were injected intradermally every other day for 2 weeks with 500 ng of IL-22 or saline in a total volume of 20 μl. (A) 6 hours after the first injection, mouse ears were harvested, RNA purified, and antimicrobial peptide transcript levels evaluated by quantitative RT-PCR. Results are reported as group means ± SEM, with n = 5 for each group. (B) H&E-stained sections 2 weeks after injection from ears injected with saline (left panel) or IL-22 (right panel). Note the keratinocyte proliferation and inflammatory infiltrates. Original magnification, ×400. (C) After 2 weeks of injections, mouse ears were harvested, RNA purified, and cytokine transcript levels evaluated by quantitative RT-PCR. Results are reported as group means ± SEM, with n = 5 for each group. Data are representative of at least 2 independent experiments.