IL-22 is required for Th17 cell–mediated pathology in a mouse model of psoriasis-like skin inflammation
J. Clin. Invest. Hak-Ling Ma, et al. 118:597 doi:10.1172/JCI33263 [
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Figure 5IL-22 neutralization influences gene expression, T cell phenotype, and cytokine profile. Recipient mice received an i.p. injection of IL-22 antibody (IL22-104) or isotype control antibody immediately before adoptive transfer and weekly thereafter. At the termination of the study, quantitative RT-PCR was performed on the RNA isolated from individual mouse ears for transcripts encoding (
A) antimicrobial peptides and (
B) cytokines. Results are reported as means ± SEM. All data are representative of at least 2 independent experiments, with
n = 10 for each group. (
C) Intracellular cytokine staining for IL-22, IL-17A, IL-17F, and IFN-γ performed on pooled cervical lymph node cells collected from mice treated with isotype control or IL-22 antibody. Results shown are gated on the CD4
+ population. Data are representative of at least 2 independent experiments. (
D) Serum was collected at the termination of a study from mice given isotype or IL-22 antibody. Serum IL-22, IL-17A, IL-17F, and IL-6 were determined by standard ELISA. Results are reported as group means ± SEM, with
n = 10 for each group. TNF-α and IFN-γ were below the limit of detection of this assay. Data are representative of at least 2 independent experiments.
