IL-22 is required for Th17 cell–mediated pathology in a mouse model of psoriasis-like skin inflammation
J. Clin. Invest. Hak-Ling Ma, et al. 118:597 doi:10.1172/JCI33263 [Go to this article.]

Figure 2
Adoptive transfer of CD4⁺CD45RB^hi T cells depleted of CD25⁺ Tregs enhances the development of skin lesions in scid/scid mice. (A) Representative images of psoriaform lesions in scid/scid mice induced by transfer of CD4⁺CD45RB^hiCD25^– T cells. The numbers correspond to the given disease severity scores. (B) Mean disease severity in mice after transfer of indicated cells or saline. Left: *P < 0.05 starting on day 21 between CD4⁺CD45RB^hiCD25^– and saline groups; ^#P < 0.05 starting on day 35 between CD4⁺CD45RB^hi and saline; **P < 0.05 starting on day 49 between CD4⁺CD45RB^hiCD25^– and CD4⁺CD45RB^hi (n = 10). Middle: Average Foxp3 gene expression in ear mRNA from indicated groups (n = 10). Right: Mean disease severity in scid/scid mice after transfer of T cells with or without indicated Treg numbers. *P < 0.05 between mice receiving 2 × 10⁵ and no Tregs; **P < 0.05 between 0.4 × 10⁵ and no Tregs. (C) H&E-stained ear (top panels) and back skin (bottom panels) sections from a mouse transferred with saline or CD4⁺CD45RB^hiCD25^– cells with parakeratosis (p), acanthosis (a), dermal inflammatory infiltrates (di), basilar papilla (bp), and epidermal microabscess (em) as indicated. Original magnification, ×400. (D) Intracellular cytokine staining was performed on pooled cervical lymph node cells collected from mice that received CD4⁺CD45RB^hiCD25^– T cells. Results shown are gated on CD4⁺ population. (E) Quantitative RT-PCR for the cytokines was performed on ear mRNA, with results reported as group means ± SEM (n = 10). Data are representative of at least 2 independent experiments.