The MEF2D transcription factor mediates stress-dependent cardiac remodeling in mice
J. Clin. Invest. Yuri Kim, et al. 118:124 doi:10.1172/JCI33255 [Go to this article.]

Figure 6
Pathological cardiac remodeling induced by overexpression of MEF2D. (A) Proteins were extracted from ventricles of α-MHC-MEF2D transgenic mouse lines at 6 weeks to 8 weeks of age for Western blot analysis using an antibody against MEF2D. GAPDH was used as a loading control. (B) Hearts were dissected from α-MHC-MEF2D male mice (line 2) and WT littermates at 8 weeks of age. Note the enlarged left atrium in the α-MHC-MEF2D heart. The lower panels are histological sections stained with Masson’s trichrome to detect fibrosis. Scale bar: 1 mm (middle panel); 40 μm (bottom panel). (C) Expression level of various hypertrophy and fibrosis markers was evaluated by quantitative PCR in each transgenic line. Note the correlation between expression level of MEF2D (A) and that of hypertrophy and fibrosis markers.