IL-22 ameliorates intestinal inflammation in a mouse model of ulcerative colitis
J. Clin. Invest. Ken Sugimoto, et al. 118:534 doi:10.1172/JCI33194 [Go to this article.]

Figure 2
Development of local gene-delivery system that is capable of targeting colon. (A) A complex of mock or enhanced GFP vector with DOTAP/enhancer reagent was locally microinjected into the proximal part (just below ileocecal junction) of colon of TCRαKO mice thorough the laparotomy approach. The mice were sacrificed 1, 2, or 4 weeks after the microinjection. Frozen sections of the injection site were subjected to fluorescent microscopic analysis for the detection of GFP signals. The result is representative of 6 individual experiments. (B) Adherent cell populations (such as macrophages and fibroblasts) were isolated from the deepithelialized mucosa with GFP vector delivery using a “walk-out” approach. The obtained cells with an adherent ability were subjected to fluorescent microscopic analysis for the detection of GFP signals. Original magnification, ×40.