The GPCR modulator protein RAMP2 is essential for angiogenesis and vascular integrity
J. Clin. Invest. Yuka Ichikawa-Shindo, et al. 118:29 doi:10.1172/JCI33022 [Go to this article.]

Figure 6
In vivo vascular permeability assay. (A) Footpad edema model. λ-Carrageenan was injected into the footpad of 8-week-old RAMP2^+/– and WT mice to induce edema for the evaluation of vascular permeability in adult mice; swelling of the footpad was measured hourly using a thickness gauge. RAMP2^+/– mice showed significantly greater swelling than WT mice. n = 12 per group. **P < 0.01, *P < 0.05 vs. WT. (B) Skin edema model (see Methods). Fluorescence intensity was measured using a fluorescence microplate reader. Permeability levels are presented relative to WT. RAMP2^+/– mice (n = 8) showed significantly greater vascular permeability than WT mice (n = 13). **P < 0.01 vs. WT. (C) Brain edema model (see Methods). Vascular permeability in RAMP2^+/– mice (n = 12) is presented relative to that in WT mice (n = 10). RAMP2^+/– mice showed significantly greater vascular permeability than WT mice. *P < 0.05 vs. WT.