Cardiomyocyte GATA4 functions as a stress-responsive regulator of angiogenesis in the murine heart
J. Clin. Invest. Joerg Heineke, et al. 117:3198 doi:10.1172/JCI32573 [
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Figure 2Induction of coronary angiogenesis by GATA4. (
A) Representative staining of histological sections in WT and DTG mice by H&E staining, Masson’s Trichrome staining (Tri), CD31 (green), and CD31 + WGA (red). Original magnification, ×100 (H&E and Tri); ×400 (CD31 and CD31 + WGA). (
B–
E) Quantification of absolute number of capillaries per microscopic field or capillaries per cardiomyocyte in control mice (Con; WT and tTA), DTG, and CnA Tg mice. *
P < 0.01 versus WT and tTA. (
F) Conductance vessels quantified by size: small (20–50 μm), medium (50–100 μm), and large (>100 μm). *
P < 0.01 versus control (WT and tTA). (
G) Coronary flow (ml/min) was measured in a working heart preparation in control (WT and tTA) and DTG mice with (2 μg/min) and without nitroprusside. *
P < 0.01 versus control vehicle;
#P < 0.01 versus control nitroprusside. (
H) Cardiac contractile function measured as +d
P/d
t (mmHg/s) in the presence (2 μg/min) or absence of nitroprusside in control and DTG mice. *
P < 0.01 versus control with nitroprusside and control and DTG without nitroprusside. Numbers inside the bars indicate the number of animals analyzed. Hearts were sectioned and multiple sections per heart were quantified.
