Factor I is required for the development of membranoproliferative glomerulonephritis in factor H–deficient mice
J. Clin. Invest. Kirsten L. Rose, et al. 118:608 doi:10.1172/JCI32525 [Go to this article.]

Figure 2
Complement profile in Cfi^–/– mice. Plasma C3 (A), factor H (B) and factor B (C) levels in Cfi^–/–, Cfi^+/–, and wild-type mice. Horizontal bars denote median values. Western blot for mouse C3 using EDTA plasma from wild-type and Cfi^–/– mice under reducing (D) and nonreducing (E) conditions. In comparison with the molecular weight of the intact C3 α-chain in wild-type plasma, the α-chain of C3 derived from Cfi^–/– plasma ran at approximately 110 kDa (D), consistent with the molecular weight of the α′-chain. Under nonreducing conditions C3c was detectable only in EDTA plasma from wild-type mice, as evident by the presence of 130-kDa bands most easily seen on high exposure (E). EDTA plasma dilutions were 1/1000 for both Cfi^–/– and wild-type mice.