Bone marrow–derived circulating progenitor cells fail to transdifferentiate into adipocytes in adult adipose tissues in mice
J. Clin. Invest. Young Jun Koh, et al. 117:3684 doi:10.1172/JCI32504 [Go to this article.]

Figure 6
No GFP⁺perilipin⁺ adipocytes were detected in regenerative adipose tissues and wound-healing dermal tissue. (A–I) A proximal portion (20% of the total length) of the left-side epididymal adipose tissue of 8-week-old C57BL/6J mice that had received BMT from GFP⁺ mice 2 months before the dissection (dotted lines, A and E). The mice were fed a high-fat diet for the indicated periods. (B–D and F–I) Both sides of the epididymal adipose tissue from the mice were harvested, photographed, and immunostained for perilipin (red stain) and PECAM-1 (blue stain) of the dissected region of the left epididymal adipose tissue (A and E, boxed regions). Some clustered GFP⁺ cells (green stain) looked like unilocular adipocytes (B and C), but the sequential dissected images of this portion (boxed region in C) revealed smaller and clustered GFP⁺perilipin^– cells (D). No GFP⁺perilipin⁺ adipocytes were detected in the adipose tissues. (J–S) Hole-punch injuries made in the ears of 8-week-old C57BL/6J mice that had received BMT from GFP⁺ mice 2 months before and were then fed a high-fat diet for the indicated times. Ears from the mice were harvested and photographed, and immunostained for perilipin and PECAM-1 at the healing region of ear (J and O, boxed regions). (K–N and P–S) No GFP⁺ cells were perilipin⁺ adipocytes in the wound-healing dermal tissues after the injury. Scale bars: 100 μm.