Bone marrow–derived circulating progenitor cells fail to transdifferentiate into adipocytes in adult adipose tissues in mice
J. Clin. Invest. Young Jun Koh, et al. 117:3684 doi:10.1172/JCI32504 [Go to this article.]

Figure 3
Clustered GFP⁺ cells in the adipose tissue of mice fed the high-fat diet were F4/80⁺ macrophages. (A) Epididymal adipose tissue was harvested from C57BL/6J mice that had received BMT from GFP⁺ mice 2 months previously and then were fed a high-fat diet for the 2-month period. Tissues were whole-mounted; coimmunostained for perilipin (red stain) and the macrophage markers CD11b, F4/80, LYVE-1, or CD45 (blue stain); and merged. Note that most of the clustered GFP⁺ cells (green stain) were F4/80⁺ macrophages, and to a lesser extent, these were CD11b⁺ or CD45⁺ macrophages. Scale bar: 100 μm. (B) GFP⁺CD11b⁺, GFP⁺F4/80⁺, GFP⁺LYVE-1⁺, and GFP⁺CD45⁺ macrophages were counted in 30 regions of clustered GFP⁺ cells (~3,000) and presented as a percentage of the total counted, clustered GFP⁺ cells. (C) Higher-magnification view of the merged GFP-, perilipin-, and F4/80-stained (green, red, and blue stain, respectively) image in A revealed characteristic GFP⁺F4/80⁺ clustered macrophages. Scale bars: 50 μm.