CP-31398 restores mutant p53 tumor suppressor function and inhibits UVB-induced skin carcinogenesis in mice
J. Clin. Invest. Xiuwei Tang, et al. 117:3753 doi:10.1172/JCI32481 [Go to this article.]

Figure 6
Effects of CP-31398 treatment on the induction of apoptosis in neonatal human epidermal keratinocytes and human epidermoid carcinoma A431 cells. (A) Western blot showing the expression of apoptosis proteins. (B) Western blot showing the expression of cell-cycle regulatory proteins in A431 cells following CP-31398 treatment. (C) Effect of CP-31398 on the induction of apoptosis and release of mitochondrial proteins in NHEKs carrying wild-type p53 and A431 cells carrying mutant p53. (D) Effect of CP-31398 on the changes in mitochondrial membrane potential in A431 cells. A431 cells were treated with PBS (control) or various concentrations of CP-31398 as indicated in the figure for different time intervals. For Western blot analysis, 100 μg protein was loaded in each well. Each experiment was repeated at least 3 times. For assaying mitochondrial membrane potential, A431 cells were treated with PBS (control) or 10, 20, and 40 μg/ml of CP-31398 for 10 minutes and stained with JC-1 dye. Carbonyl cyanide 3-chlorophenylhydrazone (CCCP) was used as positive control. Error bars represent mean ± SD of triplicate samples.