Overexpression of sPRDM16 coupled with loss of p53 induces myeloid leukemias in mice
J. Clin. Invest. Danielle C. Shing, et al. 117:3696 doi:10.1172/JCI32390 [Go to this article.]

Figure 5
Competitive reconstitution assay. (A) Experimental approach. Lethally irradiated recipient mice (CD45.1) were competitively repopulated with 300,000 test lin^– cells (CD45.2 WT or p53^–/–, transduced with MSCV or sPRDM16), 300,000 competitor lin^– cells (CD45.1 WT, transduced with MSCV) and 500,000 spleen cells (CD45.1 WT). For each group, 4–5 mice were reinoculated, and peripheral blood was analyzed by FACS analysis at 4 and 8 weeks after transplantation. (B and C) Relative contributions of test (CD45.2) versus competitor (CD45.1) populations in peripheral blood of mice at 4 and 8 weeks after transplantation. Flow cytometry of representative mice (B) and mean contributions for each group of 4–5 mice (C) are shown. (D) The proportion of each lineage derived from donor test (CD45.2) cells is shown in the peripheral blood of mice at 4 and 8 weeks after transplantation.