Overexpression of sPRDM16 coupled with loss of p53 induces myeloid leukemias in mice
J. Clin. Invest. Danielle C. Shing, et al. 117:3696 doi:10.1172/JCI32390 [Go to this article.]

Figure 3
sPRDM16 overexpression in lin^– cells. (A) PRDM16 and sPRDM16 cDNAs were cloned into the MSCV retroviral vector at its EcoRI cloning site (E). (B) Anti-PRDM16 immunofluorescence of transduced lin^– cells. (C–E) Serial replating of transduced WT and p53^–/– lin^– cells in methylcellulose, in the presence of cytokines G-CSF and GM-CSF (G, GM). Cell morphology before differentiation stimuli and at the third and fourth platings (MGG stain; C), CFUs upon serial replating (D), and micrographs at the third plating (E) are shown. Original magnification, ×1,000 (B); ×400 (C).