Overexpression of sPRDM16 coupled with loss of p53 induces myeloid leukemias in mice
J. Clin. Invest. Danielle C. Shing, et al. 117:3696 doi:10.1172/JCI32390 [Go to this article.]

Figure 2
Expression patterns of PRDM16. (A) Positions of primers used to detect expression of PRDM16 alone or both PRDM16 and sPRDM16 by SYBR Green (green arrows) and Taqman (blue arrows) Q-PCR along PRDM16 and sPRDM16 transcripts (red arrows). (B and C) Q-PCR of PRDM16 expression in AMLs with (B) or without (C) 1p36 rearrangements. Expression was normalized to GAPDH and calibrated to the median level in 10 normal CD34⁺ cells (shown individually in C). AMLs are grouped according to karyotype: common translocations [t(15;17), t(8;21), inv(16), 11q23, t(9;22)], rare translocation, or normal karyotype. AML-NK cases are grouped according to the presence of NPM or FLT3-ITD mutations.