Overexpression of sPRDM16 coupled with loss of p53 induces myeloid leukemias in mice
J. Clin. Invest. Danielle C. Shing, et al. 117:3696 doi:10.1172/JCI32390 [
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Figure 1PRDM16 translocations in 5 leukemia cases.
(
A) Genomic breakpoint regions (double-headed arrows) at 1p36 of cases 1–5, defined by FISH mapping.
PRDM16 exons are shown in red, together with ATG start sites of PRDM16 and sPRDM16 in exons 1 and 4, respectively. The position of the bacterial artificial chromosome (BAC) clones (upstream of and spanning
PRDM16) used as FISH probes to map the breakpoint regions of the 5 leukemias are shown in blue. (
B–
E) Predicted genomic rearrangement and transcription products for cases 1–5, defined by FISH, 5′-RACE, and RT-PCR. Grey and orange arrows indicate primer positions for 5′-RACE and RT-PCR, respectively. Asterisks indicate novel rearrangements.
