Ang II–stimulated migration of vascular smooth muscle cells is dependent on LR11 in mice
J. Clin. Invest. Meizi Jiang, et al. 118:2733 doi:10.1172/JCI32381 [Go to this article.]

Figure 4
Ang II–induced membrane ruffle formation of cultured SMCs derived from Lr11^–/– mice. (A–L) Ang II–induced membrane ruffling (arrowheads) in Lr11^+/+ or Lr11^–/– SMCs. The indicated SMCs were incubated with or without Ang II (1 μM) for 24 hours in the presence or absence of recombinant sLR11 (1 μg/ml) with or without anti-uPAR antibody for 24 hours. PDGF-BB (10 ng/ml) was then added to the culture medium for 10 minutes before immunofluorescence analyses. Cells were then stained using Alexa Fluor 488 phalloidin. Scale bars: 10 μm. (M) The number of cells with membrane ruffles were counted among 500 cells in the field. Data are presented as mean ± SD (n = 3). *P < 0.05. ND, not detected.